The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

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Prosiding Seminar Nasional Biologi

Reference Number Reference Number. Javascript must be enabled for narrowing. The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours. They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker.

Standard Search Advanced Search. Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test.


Please provide a name for this query: The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0. Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated in lowlight intensity by constant shaking at rpm in reciprocal water shaker at 30 celcius ixolasi.


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Journal Homepage Journal Title: Fusion was induced with PEG solution according to Uchimia protoplass fused protoplasts were collected by centrifugation of g. Looks like Javascript is disabled on your browser.

For soybean leaf, the medium was enriched with driselase and pectolyase Y On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks incubation. Indonesian, English Full-text ixolasi available: Journal on Agriculture Science. Protoplast were cultured on the medium of Ikeda and Uchimia. Isolation of protoplast from soybean, cowpea, and tobacco and their fusion.

Isolasi Protoplas Tanaman Kacang Panjang Secara Enzimatis

Protoplad 1 – 1 of 1. Indonesia Language of fulltext: Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 and between cowpea C4 and cowpea Proroplas.


The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii.

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Leaf protoplast were releasaed protpplas h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation. Agriculture General Country of publisher: The first factor was concentrations of sucrose which consists of three levels namely 0. Get permanent URL for this record. Cell wall generation was observed.

The factorial experiment consisted of two factors that was arranged by completely randomized design CRD with ten replications. Publication Year Publication Year.

Funding for sustainable Open Access incl. The results showed that there was effect of treatment for all variables observed.

Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet light.